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Prediction of thioredoxin and glutaredoxin target proteins by identifying reversibly oxidized cysteinyl residues

Lee, Hang-Mao ; Dietz, Karl J. ; Hofest├Ądt, Ralf

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)


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Abstract:
A significant part of cellular proteins undergo reversible thiol-dependent redox transitions which often control or switch protein functions. Thioredoxins and glutaredoxins constitute two key players in this redox regulatory protein network. Both interact with various categories of proteins containing reversibly oxidized cysteinyl residues. The identification of thioredoxin/glutaredoxin target proteins is a critical step in constructing the redox regulatory network of cells or subcellular compartments. Due to the scarcity of thioredoxin/glutaredoxin target protein records in the public database, a tool called Reversibly Oxidized Cysteine Detector (ROCD) is implemented here to identify potential thioredoxin/glutaredoxin target proteins computationally, so that the in silico construction of redox regulatory network may become feasible. ROCD was tested on 46 thioredoxin target proteins in plant mitochondrion, and the recall rate was 66.7% when 50% sequence identity was chosen for structural model selection. ROCD will be used to predict the thioredoxin/glutaredoxin target proteins in human liver mitochondrion for our redox regulatory network construction project. The ROCD will be developed further to provide prediction with more reliability and incorporated into biological network visualization tools as a node prediction component. This work will advance the capability of traditional database- or text mining-based method in the network construction.


Institution: Faculty of Technology, Research Groups in Informatics
DDC classification: Data processing, computer science, computer systems

Suggested Citation:
Lee, Hang-Mao ; Dietz, Karl J. ; Hofestädt, Ralf  (2010)  Prediction of thioredoxin and glutaredoxin target proteins by identifying reversibly oxidized cysteinyl residues. Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 7(3), 2010

Online-Journal: http://journal.imbio.de/article.php?aid=130
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2010/5030



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