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Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters

Olivieri, David ; Faro, José ; Gómez-Conde, Iván ; Tadokoro, Carlos E.

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)


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Abstract:
This paper describes a novel software algorithm, called constrained Sequential Monte Carlo (SMC) clusters, for tracking a large collection of individual cells from intra-vital two-photon microscopy image sequences. We show how our method and software tool, implemented in python, is useful for quantifying the motility of T and B lymphocytes involved in an immune response vs lymphocytes under non immune conditions. We describe the theory behind our algorithm and briefly discuss the architecture of our software. Finally, we demonstrate both the functionality and utility of software by applying it to two practical examples from videos displaying lymphocyte motility in B cell zones (follicles) and T cell zones of lymph nodes.


Institution: Faculty of Technology, Research Groups in Informatics
DDC classification: Data processing, computer science, computer systems

Suggested Citation:
Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters. Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 8(3), 2011

Online-Journal: http://journal.imbio.de/article.php?aid=180
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2011/5196



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