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On Comparison of SimTandem with State-of-the-Art Peptide Identification Tools, Efficiency of Precursor Mass Filter and Dealing with Variable Modifications

Novak, Jiri ; Sachsenberg, Timo ; Hoksza, David ; Skopal, Tomas ; Kohlbacher, Oliver

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)


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Abstract:
The similarity search in theoretical mass spectra generated from protein sequence databases is a widely accepted approach for identification of peptides from query mass spectra produced by shotgun proteomics. Growing protein sequence databases and noisy query spectra demand database indexing techniques and better similarity measures for the comparison of theoretical spectra against query spectra. We employ a modification of previously proposed parameterized Hausdorff distance for comparisons of mass spectra. The new distance outperforms the original distance, the angle distance and state-of-the-art peptide identification tools OMSSA and X!Tandem in the number of identified peptides even though the q-value is only 0.001. When a precursor mass filter is used as a database indexing technique, our method outperforms OMSSA in the speed of search. When variable modifications are not searched, the search time is similar to X!Tandem. We show that the precursor mass filter is an efficient database indexing technique for high-accuracy data even though many variable modifications are being searched. We demonstrate that the number of identified peptides is bigger when variable modifications are searched separately by more search runs of a peptide identification engine. Otherwise, the false discovery rates are affected by mixing unmodified and modified spectra together resulting in a lower number of identified peptides. Our method is implemented in the freely available application SimTandem which can be used in the framework TOPP based on OpenMS.


Institution: Faculty of Technology, Research Groups in Informatics
DDC classification: Data processing, computer science, computer systems

Suggested Citation:
Novak, Jiri ; Sachsenberg, Timo ; Hoksza, David ; Skopal, Tomas ; Kohlbacher, Oliver  (2013)  On Comparison of SimTandem with State-of-the-Art Peptide Identification Tools, Efficiency of Precursor Mass Filter and Dealing with Variable Modifications. Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 10(3): Special Issue: Selected extended papers of the 7th International Conference on Practical Applications of Computational Biology and Bioinformatics, Salamanca, Spain, 2013

Online-Journal: http://journal.imbio.de/article.php?aid=228
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2013/5306



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