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A distributed computational search strategy for the identification of diagnostics targets: Application to finding aptamer targets for methicillin-resistant staphylococci

Flanagan, Keith ; Cockell, Simon ; Harwood, Colin ; Hallinan, Jennifer ; Nakjang, Sirintra ; Lawry, Beth ; Wipat, Anil

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)



Abstract:
The rapid and cost-effective identification of bacterial species is crucial, especially for clinical diagnosis and treatment. Peptide aptamers have been shown to be valuable for use as a component of novel, direct detection methods. These small peptides have a number of advantages over antibodies, including greater specificity and longer shelf life. These properties facilitate their use as the detector components of biosensor devices. However, the identification of suitable aptamer targets for particular groups of organisms is challenging. We present a semi-automated processing pipeline for the identification of candidate aptamer targets from whole bacterial genome sequences. The pipeline can be configured to search for protein sequence fragments that uniquely identify a set of strains of interest. The system is also capable of identifying additional organisms that may be of interest due to their possession of protein fragments in common with the initial set. Through the use of Cloud computing technology and distributed databases, our system is capable of scaling with the rapidly growing genome repositories, and consequently of keeping the resulting data sets up-to-date. The system described is also more generically applicable to the discovery of specific targets for other diagnostic approaches such as DNA probes, PCR primers and antibodies.


Beteiligte Einrichtung: Technische Fakultät, Arbeitsgruppen der Informatik
DDC-Sachgruppe: Datenverarbeitung, Informatik

Zitat-Vorschlag:
Flanagan, Keith ; Cockell, Simon ; Harwood, Colin ; Hallinan, Jennifer ; Nakjang, Sirintra ; Lawry, Beth ; Wipat, Anil  (2014)  A distributed computational search strategy for the identification of diagnostics targets: Application to finding aptamer targets for methicillin-resistant staphylococci. Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 11(2): Special Issue: 10th International Symposium on Integrative Bioinformatics, Newcastle, United Kingdom, 2014

Online-Journal: http://journal.imbio.de/article.php?aid=242
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2014/5323



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 Letzte Änderung: 15.2.2011
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