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UV-visible scanning spectrophotometry and chemometric analysis as tools for carotenoids analysis in cassava genotypes (Manihot esculenta Crantz)

Moresco, Rodolfo ; Gavicho Uarrota, Virgi­lio ; Pereira, Aline ; Tomazzoli, Mai­ra Maciel ; Da Costa Nunes, Eduardo ; Martins Peruch, Luiz Augusto ; Gazzola, Jussara ; Costa, Christopher ; Rocha, Miguel ; Maraschin, Marcelo

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)



Abstract:
In this study, the metabolomics characterization focusing on the carotenoid composition of ten cassava (Manihot esculenta) genotypes cultivated in southern Brazil by UV-visible scanning spectrophotometry and reverse phase-high performance liquid chromatography was performed. Cassava roots rich in β-carotene are an important staple food for populations with risk of vitamin A deficiency. Cassava genotypes with high pro-vitamin A activity have been identified as a strategy to reduce the prevalence of deficiency of this vitamin. The data set was used for the construction of a descriptive model by chemometric analysis. The genotypes of yellow-fleshed roots were clustered by the higher concentrations of cis-β-carotene and lutein. Inversely, cream-fleshed roots genotypes were grouped precisely due to their lower concentrations of these pigments, as samples rich in lycopene (redfleshed) differed among the studied genotypes. The analytical approach (UV-Vis, HPLC, and chemometrics) used showed to be efficient for understanding the chemodiversity of cassava genotypes, allowing to classify them according to important features for human health and nutrition.


Beteiligte Einrichtung: Technische Fakultät, Arbeitsgruppen der Informatik
DDC-Sachgruppe: Datenverarbeitung, Informatik

Zitat-Vorschlag:
Moresco, Rodolfo ; Gavicho Uarrota, Virgi­lio ; Pereira, Aline ; Tomazzoli, Mai­ra Maciel ; Da Costa Nunes, Eduardo ; Martins Peruch, Luiz Augusto ; Gazzola, Jussara ; Costa, Christopher ; Rocha, Miguel ; Maraschin, Marcelo  (2015)  UV-visible scanning spectrophotometry and chemometric analysis as tools for carotenoids analysis in cassava genotypes (Manihot esculenta Crantz). Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 12(4): Special Issue: Selected extended papers of the 9th International Conference on Practical Applications of Computational Biology and Bioinformatics, Salamanca, Spain, 2015. Guest editors: Florentino Fdez-Riverola, Juan F. De Paz, Ross Overbeek and Miguel Rocha

Online-Journal: http://journal.imbio.de/
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2015/5419



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 Letzte Änderung: 23.06.2015
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