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Online High-throughput Mutagenesis Designer Using Scoring Matrix of Sequence-specific Endonucleases

Guo, Dayong ; Li, Xiaojing ; Zhu, Pan ; Feng, Yanrong ; Yang, Juan ; Zheng, Zhihong ; Yang, Wei ; Zhang, Enuo ; Yu, Yang ; Zhou, Shenglai ; Wang, Hongyu

Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516)



Abstract:
CRISPR Cas9 and other sequence-specific endonucleases are fundamental genome editors supporting gene knockout and gene therapy. A speedy and accurate computational allele designer is required for a high through-put gene mutagenesis pipeline using these new techniques. An automatic system, Cas9 online designer (COD), was created to screen Cas9 targets and off-targets, as well as to provide gene knockout and genotyping strategies. A gene knockout rat model was successfully created and genotyped under the direction of this online system confirming its ability to predict real targets and off-targets. Gene knockout strategies to mutate 72 rat cytochrome P450 genes were designed instantly by the system to demonstrate its high-throughput efficiency. Also, the system used an off-target scoring matrix which can be applied to any sequence-specific genome editing tools besides Cas9. The COD system (http://cas9.wicp.net) has established a speedy, accurate, flexible and high through-put computational gene knockout pipeline supporting the sequence-specific endonuclease induced mutagenesis.


Beteiligte Einrichtung: Technische Fakultät, Arbeitsgruppen der Informatik
DDC-Sachgruppe: Datenverarbeitung, Informatik

Zitat-Vorschlag:
Guo, Dayong ; Li, Xiaojing ; Zhu, Pan ; Feng, Yanrong ; Yang, Juan ; Zheng, Zhihong ; Yang, Wei ; Zhang, Enuo ; Yu, Yang ; Zhou, Shenglai ; Wang, Hongyu  (2015)  Online High-throughput Mutagenesis Designer Using Scoring Matrix of Sequence-specific Endonucleases. Journal of Integrative Bioinformatics - JIB (ISSN 1613-4516), 12(1), 2015

Online-Journal: http://journal.imbio.de/
URL: http://biecoll.ub.uni-bielefeld.de/volltexte/2015/5422



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 Letzte Änderung: 23.06.2015
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